Identification of antibiotic resistance genes
In recent decades, there has been a rapid spread of antibiotic resistance (ABR) among infectious disease pathogens. Development of ABR results in microorganisms' ability to survive despite the application of targeted therapy. ABR hinders effective patient treatment and contributes to the formation of chronic, recurrent infections.
Molecular genetic assay
Molecular genetic detection of antibiotic resistance genes in biological samples can be used alongside phenotypic methods to initiate targeted therapy even before extracting the pathogen in pure culture. In cases of an unclear inhibition zone boundary during microbiological testing, PCR-based diagnostics for antibiotic resistance is also recommended. When prescribing treatment for critically ill patients, children, elderly individuals, and immunocompromised persons, PCR testing for resistance genes can be combined with microbiological culture and antibiotic susceptibility testing.
Detection of antibiotic resistance genes provides crucial additional clinical information for physicians when selecting effective therapy – information that cannot be obtained through microbiological susceptibility testing alone. PCR diagnostics is a direct method that enables the identification of antibiotic resistance genes in difficult-to-culture microorganisms. The results of PCR testing are not affected by the storage conditions of antibacterial drugs and culture media.
PCR advantages
- Rapid, supplementary information on the presence of antibiotic resistance genes in bacteria, enabling faster selection of optimal antibacterial therapy.
- The use of instrumental PCR methodology ensures reproducible results independent of the specific setup of a microbiology laboratory.
- Capability to detect antibiotic resistance in difficult-to-cultivate organisms.
- Ability to analyze a wide range of biological samples.
- High analytical sensitivity.
Indications
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Lack of response to antibacterial therapy -
Infectious complications in oncology patients -
Infectious complications in hospitalized patients -
Presence of invasive devices (endotracheal tube, catheters, drains)
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Compromised skin integrity: wounds, burns, pressure ulcers -
Surgical site infections -
Infections of the upper and lower respiratory and urinary tracts -
Respiratory infections in cystic fibrosis patients
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Immunodeficiency conditions -
Neonatal infections -
Fever of unknown origin
List of PCR studies
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BacResista GLA, BacResista GLA Van/Mec
A test to identify glycopeptide and beta-lactam antibiotic resistance genes in DNA derived from bacterial cultures found in human biological material.
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Please note that the specialists of the DNA Technology company provide consultations exclusively to medical specialists on the application and research features. Requests related to the appointment, delivery, or interpretation of tests are not considered. For relevant information, we recommend contacting the laboratory directly.