Differential diagnosis of acute respiratory infections, including influenza and COVID-19, is necessary to assess the severity of the course of the disease and to predict complications, as well as to prevent hospital-acquired infection. Diagnosis of respiratory syncytial infection, which causes lesions of the lower respiratory system, is of particular clinical importance in young children.
SARS-CoV-2
SARS-CoV-2 is an RNA-containing coronavirus that was first identified in 2019. It is the causative agent of COVID-19 infection. The virus targets airway epithelial, alveolar and endothelial cells.
The incubation period of COVID-19 ranges from 2 to 14 days. Usually, the symptoms appear on days 4-6 after contracting the virus. The clinical picture in most cases is identical to other acute viral respiratory infections: pain on swallowing, rhinitis, cough, fever, shivering, headaches and muscle pain. The severe form of the disease proceeds with pronounced dyspnea and hypoxia. Some patients develop hypercoagulation syndrome with thrombosis and thromboembolism; other organs and systems are also affected (CNS, myocardium, kidneys, liver, gastrointestinal tract, endocrine and immune systems), sepsis and septic shock may also develop.
Respiratory syncytial virus
It is an RNA-containing virus with tropism for the lower respiratory tract epithelium. It causes a wide range of respiratory diseases, including rhinitis, pharyngitis, bronchiolitis and pneumonia. The incubation period of the disease ranges from 2 to 8 days. Symptoms of infection may include runny nose, cough, sore throat, fever and headache. Complications from the infection may manifest as pneumothorax, atelectasis and pulmonary emphysema, bronchial asthma, and bacterial infection. Children, especially infants and toddlers, may develop a severe course of infection characterized by dyspnea and central cyanosis.
Influenza viruses A and B
Influenza viruses selectively affect epithelial cells of the respiratory tract (mainly trachea), disrupt the immune system, contribute to mixed and bacterial infections, leading to complications, like pneumonia (of primary viral, secondary bacterial or bacterial-viral forms), otitis and sinusitis. Symptoms of the disease appear 1-4 days after infection and persist for an average of one week.
Influenza A virus
Influenza A virus is one of the main pathogens of seasonal influenza outbreaks due to high variability of the virus. These viruses are classified into subtypes according to combinations of proteins (hemagglutinin and neuraminidase) on the virus surface. Currently, the most common subtypes are A(H1N1) and A(H3N2). Symptoms of the disease include fever, cough, runny nose, sore throat, muscle and joint pain, general weakness and headache.
Influenza B virus
Type B influenza viruses are found only in human populations. They do not cause pandemics, but can underlie large outbreaks and epidemics. The virus affects the upper and lower respiratory tract, and the clinical symptoms of infection are similar to those caused by influenza A virus.
Indications for testing
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Sore throat;
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Fever;
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Muscle pain;
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Cough
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Runny nose
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Contact with an AVRI patient;
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Being in foci of infection;
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Differential diagnosis.
SARS-CoV-2/RSV/ Influenza AB virus Multiplex
The SARS-CoV-2/RSV/Influenza A virus/Influenza B virus Multiplex REAL-TIME PCR Detection Kit is designed to detect RNA of coronavirus SARS-CoV-2, human respiratory syncytial virus (RSV), influenza A virus and influenza B virus in human biological samples by reverse transcription and real-time polymerase chain reaction (RT-PCR).
Sample: Nasopharyngeal swabs, oropharyngeal swabs, bronchoalveolar lavage, endotracheal aspirate, nasopharyngeal aspirate, phlegm.
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