C.trachomatis/T.vaginalis Multiplex
A test for detecting Chlamydia trachomatis and Trichomonas vaginalis DNA in human biomaterial by real-time PCR.
Category «Urogenital infections»
Chlamydia trachomatis
Chlamydia trachomatis is an obligate intracellular pathogen. It is the most common etiologic factor of bacterial STIs.
C. trachomatis penetrates the epithelial cells of the urogenital tract using specialized proteins for adhesion and invasion. Upon entry into the cell, the bacterium transforms from an elementary body to a reticular cell capable of growth and division. The reticular cells are further transformed into next-generation elementary bodies, which are then released from the cell to infect new cells, causing an inflammatory process.
C. trachomatis causes urogenital chlamydia. The most common clinical manifestation include discharge from the urethra and/or genital tract in women; in men, sporadic urethral discharge may be present. The disease is often asymptomatic, which increases the risk of transmission and complications such as pelvic inflammatory disease (PID) in women, chronic prostatitis in men, and infertility in both sexes. PID is associated with an increased risk of adhesions, chronic pelvic pain syndrome and ectopic pregnancy. Chlamydia infection in pregnant women can lead to abortion and miscarriage, placental insufficiency, intrauterine infection of the fetus, postpartum inflammatory diseases, and neonatal infections.
Trichomonas vaginalis
T. vaginalis attaches to epithelial cells of the urogenital tract using its flagella and specific adhesin proteins. Trichomonads produce cytotoxic chemicals such as cell disengaging factor and N-nitrosamines, which stimulate the development of epithelial atypia and dysplasia.
Trichomonas vaginalis is a flagellated protozoan that causes one of the most prevalent non-gonococcal infections - trichomoniasis.
Urogenital trichomoniasis in women is usually associated with the development of vulvovaginitis and cervicitis, less commonly – with urethritis. The disease is characterized by symptoms such as malodorous discharge from the genital tract, itching, burning sensations, pain on urination and during intercourse. In case of an ascending infection, PIDs may develop. In men, the epithelium of the urethral mucous membrane is primarily infected, after which trichomonads can spread to the prostate tissue, seminal vesicles. The most common symptoms are urethral discharge and pain on urination. Urogenital trichomoniasis is associated with complications such as ectopic pregnancy, preterm labor, low birth weight, and infertility in both sexes.
Comprehensive testing for Chlamydia trachomatis and Trichomonas vaginalis has important advantages:
- simultaneous detection of several pathogens is of high clinical significance, since urogenital tract infections are frequently caused by a combination of pathogens;
- accurate identification of pathogens, which enables timely prescription of etiotropic treatment;
- time saving: multiplex PCR aids prompt etiologic and differential diagnosis, even in case of asymptomatic infections.
Indications for testing
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signs of inflammation in the urogenital tract -
redness, itching, swelling -
foul odor -
reproductive dysfunction -
preconception checkup, including preparation for IVF -
evaluation of therapy effectiveness -
pelvic surgery -
an STI-positive sexual partner
C.trachomatis/T.vaginalis Multiplex
The C.trachomatis/T.vaginalis Multiplex REAL-TIME PCR Detection Kit is an in vitro Nucleic Acid Test (NAT) – pathogen-detection-based product. The C.trachomatis/T.vaginalis Multiplex REAL-TIME PCR Detection Kit is intended for simultaneous detection of Chlamydia trachomatis and Trichomonas vaginalis DNA in human biological material.
Sample: urine, scrapes of epithelial cells from the urogenital tract.
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Please note that the specialists of the DNA Technology company provide consultations exclusively to medical specialists on the application and research features. Requests related to the appointment, delivery, or interpretation of tests are not considered. For relevant information, we recommend contacting the laboratory directly.