PCR diagnostics of nosocomial and community-acquired infections.
Different departments within healthcare institutions are characterized by a distinct composition of hospital-acquired flora, with a predominance of specific microorganism species that can cause infections of varying localization and severity. They can also be the source of community-acquired infections, for example, those affecting the upper and lower respiratory and urinary tracts, skin and soft tissues, etc. Such infections can be particularly severe in patients with various underlying chronic conditions.
Causes of nosocomial and community-acquired infections
The cause of both nosocomial and community-acquired infections is often opportunistic pathogens (OPs), which exhibit antibiotic resistance. Resistance to antibacterial drugs hinders effective therapy and contributes to the emergence of chronic and recurrent infections. Diseases caused by antibiotic-resistant strains are characterized by a prolonged course, frequently extended hospital stays, and an unfavorable prognosis for patients. OPs cause infectious diseases with similar clinical presentations, making it impossible to definitively identify the causative agent without laboratory testing.
Real-time PCR is a highly sensitive method that provides rapid results, which is especially critical for treating severe infections, as the effectiveness of targeted therapy largely depends on the timely administration of appropriate antibiotics. Furthermore, PCR testing does not require handling live bacterial cultures, thereby preventing the spread and circulation of microorganisms within healthcare and laboratory facilities.
Multiplex PCR testing
For effective diagnosis of nosocomial and community-acquired infections and timely prescription of optimal therapy, it is recommended to perform the BacScreen OM PCR test in conjunction with the BacResista GLA / BacResista GLA Van/Mec test. BacScreen OM enables the identification of opportunistic pathogens down to a defined taxonomic level and determines the proportion of each pathogen in the DNA sample. The reports of BacResista GLA and BacResista GLA Van/Mec (a shortened variant of the test) provide information on the presence of antibiotic resistance genes in bacteria, which facilitates a quicker selection of optimal antibacterial therapy.
Indications
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Infectious complications in hospitalized patients -
Infectious complications in oncology patients -
Presence of invasive devices (endotracheal tube, catheters, drains) -
Compromised skin integrity: wounds, burns, pressure ulcers -
Surgical site infections -
Infections of the upper and lower respiratory and urinary tracts -
Respiratory infections in cystic fibrosis patients -
Immunodeficiency conditions -
Neonatal infections -
Fever of unknown origin
List of PCR studies
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BacScreen-OM
A test for detecting DNA of opportunistic bacteria of Bacilli, Betaproteobacteria and Gammaproteobacteria classes causing nosocomial and out-of-hospital infections.
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Please note that the specialists of the DNA Technology company provide consultations exclusively to medical specialists on the application and research features. Requests related to the appointment, delivery, or interpretation of tests are not considered. For relevant information, we recommend contacting the laboratory directly.