C.trachomatis/ N.gonorrhoeae Multiplex
A test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae DNA in human biomaterial by real-time PCR.
Category «Urogenital infections»
Chlamydia trachomatis
Chlamydia trachomatis is an obligate intracellular pathogen, the most common etiologic agent of sexually transmitted bacterial infections. C. trachomatis penetrates the epithelial cells of the urogenital tract using specialized proteins for adhesion and invasion. Upon entry into the cell, the bacterium transforms from an elementary body to a reticular cell capable of growth and division. The reticular cells are further transformed into next-generation elementary bodies, which are then released from the cell to infect new cells, causing an inflammatory process.
C. trachomatis causes urogenital chlamydia. The most common clinical manifestation include discharge from the urethra and/or genital tract in women; in men, sporadic urethral discharge may be present. The disease is often asymptomatic, which increases the risk of transmission and complications such as pelvic inflammatory disease (PID) in women, chronic prostatitis in men, and infertility in both sexes. PID is associated with an increased risk of adhesions, chronic pelvic pain syndrome and ectopic pregnancy. Chlamydia infection in pregnant women can lead to abortion and miscarriage, placental insufficiency, intrauterine infection of the fetus, postpartum inflammatory diseases, and neonatal infections.
Neisseria gonorrhoeae
Neisseria gonorrhoeae is a Gram-negative, non-spore-forming, non-motile bacteria that causes gonococcal infection. This microorganism has many pathogenicity factors, including pili, which allow adhesion to epithelial cells, and various proteases that cleave secretory immunoglobulins, thereby preventing a full-scale immune response.
The most common clinical manifestations of gonococcal infection in women are urethral and/or genital discharge, itching, burning sensation, and pain on urination and sexual intercourse. In men, urethral discharge, itching, burning sensation, and pain on urination may be present. Chronic infection may cause infertility in both sexes. In case of an ascending infection, PIDs may develop. Gonococcal infection during pregnancy may be associated with adverse birth outcomes, including preterm labor, premature rupture of the fetal membranes, low birth weight, neonatal ophthalmia, and perinatal mortality. Extragenital forms of the disease may affect the oropharyngeal mucosa, the rectum, and the conjunctiva.
Comprehensive testing for Chlamydia trachomatis and Neisseria gonorrhoeae has important advantages:
- simultaneous detection of several pathogens is of high clinical significance, since urogenital tract infections are frequently caused by a combination of pathogens;
- accurate identification of pathogens, which enables timely prescription of etiotropic treatment;
- time saving: multiplex PCR aids prompt etiologic and differential diagnosis, even in case of asymptomatic infections.
Indications for testing
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signs of inflammation in the urogenital tract -
redness, itching, swelling -
foul odor -
reproductive dysfunction -
preconception checkup, including preparation for IVF -
evaluation of therapy effectiveness -
pelvic surgery -
an STI-positive sexual partner
C.trachomatis/ N.gonorrhoeae Multiplex
The C.trachomatis/N.gonorrhoeae Multiplex REAL-TIME PCR Detection Kit is an in vitro Nucleic Acid Test (NAT) – pathogen-detection-based product. The C.trachomatis/N.gonorrhoeae Multiplex REAL-TIME PCR Detection Kit is intended for simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae DNA in human biological material.
Sample: urine, scrapes of epithelial cells from the urogenital tract.
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Please note that the specialists of the DNA Technology company provide consultations exclusively to medical specialists on the application and research features. Requests related to the appointment, delivery, or interpretation of tests are not considered. For relevant information, we recommend contacting the laboratory directly.