C.pneumoniae/M.pneumoniae Multiplex
The C.pneumoniae/M.pneumoniae Multiplex test for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae DNA in human biological material by real-time PCR.
Category «Respiratory tract infections»
C.pneumoniae/M.pneumoniae Multiplex
Chlamydophila pneumoniae is an obligate intracellular Gram-negative bacterium and one of the most common respiratory pathogens.
Mycoplasma pneumoniae pneumoniae is a small Gram-negative bacterium that lacks a rigid cell wall. This structural feature renders mycoplasmas resistant to many classes of antibiotics that inhibit cell wall synthesis (e.g., beta-lactams). M. pneumoniae, like C. pneumoniae, is among the most common infectious agents causing upper and lower respiratory tract infections, particularly in children and young adults.
Both pathogens are transmitted via the airborne route through close contact with an infected individual. The sources of infection include symptomatic patients, asymptomatic carriers, and convalescent individuals (including those who have completed a course of antibacterial therapy).
The intracellular localization and ability to parasitize eukaryotic cell membranes allow M. pneumoniae and C. pneumoniae to evade an active immune response and ensure the persistence of these pathogens in the human body.
C. pneumoniae and M. pneumoniae can persist asymptomatically for long periods in the epithelial cells of the respiratory tract, accounting for 20–30% of the etiological structure of respiratory infections. In some cases, clinical manifestation of the disease occurs several months after infection. Infectious–inflammatory lung damage caused by C. pneumoniae and M. pneumoniae is classified as atypical pneumonia and is characterized by less pronounced respiratory symptoms with a predominance of the intoxication syndrome.
The list of caused pathological processes
Delayed administration of targeted therapy contributes to a prolonged and recurrent disease course and facilitates the spread of infection.
M. pneumoniae and C. pneumoniae cause various pathological processes of the upper and lower respiratory tract:
- Acute respiratory diseases affecting the upper respiratory tract: nasopharyngitis, laryngitis
- Acute respiratory diseases affecting the lower respiratory tract: tracheobronchitis; a persistent, often non-productive cough that may last for several weeks;
- Community-acquired (atypical) pneumonia: characterized by gradual onset, dry cough, headache and muscle pain, and low-grade fever;
- Exacerbation of bronchial asthma and chronic obstructive pulmonary disease (COPD).
In cases of severe inflammation, chronic interstitial pulmonary fibrosis may develop. Severe infection can lead to a wide range of complications affecting the cardiovascular system (myocarditis, pericarditis), the nervous system (meningoencephalitis, serous meningitis, myelitis, and others), the musculoskeletal system (arthritis of various locations), and the hematopoietic system (hemolytic anemia). The role of M. pneumoniae in the development of various immunopathological conditions (rheumatoid arthritis, Guillain–Barré syndrome, erythema multiforme, Stevens–Johnson syndrome, and others) is being studied.
A multiplex PCR test designed for simultaneous detection of C. pneumoniae and M. pneumoniae offers high sensitivity and specificity, provides rapid results, enables differential diagnosis, and facilitates the initiation of targeted therapy.
Indications
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Differential diagnostics of acute respiratory infections (including atypical pneumonia) -
Cough of unclear origin -
Examination during exacerbation of bronchial asthma and/or COPD to identify an infectious trigger -
Monitoring of antibacterial therapy effectiveness
C.pneumoniae/M.pneumoniae Multiplex
The C.pneumoniae/M.pneumoniae Multiplex REALTIME PCR Detection Kit is designed to detect DNA of Chlamydophila pneumoniae and Mycoplasma pneumoniae in human biological material by real-time PCR.
Sample: Nasopharyngeal and oropharyngeal smears, bronchoalveolar lavage, endotracheal and nasopharyngeal aspirate, phlegm, pleural fluid
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Please note that the specialists of the DNA Technology company provide consultations exclusively to medical specialists on the application and research features. Requests related to the appointment, delivery, or interpretation of tests are not considered. For relevant information, we recommend contacting the laboratory directly.